Area of focus: Therapeutics/Immunology Commencement Date: May, 2017 Location: JLABS @ San Diego
The natural antibody response to immunological challenge is a rich source of therapeutic candidates. Current technologies, such as hybridoma and phage display, provide a limited and biased view into the diverse antibody repertoire. Our goal is to interrogate the repertoire directly to rapidly identify high-affinity antibodies for therapeutic and diagnostic applications.
Currently, antibody repertoire analysis utilizes next-generation sequencing of antibody transcripts from B-cells. Recent studies have shown that these repertoires are a poor proxy for the collection of antibodies in serum. We leverage our expertise and intellectual property in mass spectrometry-based proteomics to deeply sample the antibodies present in serum. We corroborate the proteomic data with the next-generation sequencing-derived repertoire, and we further employ sophisticated spectrum analysis algorithms to identify novel antibody clones that were missed by next-generation sequencing, increasing the serum repertoire diversity by as much as 50%.
We are driven to make antibody discovery more efficient in order to accelerate clinical development.